Biophysique des membranes et RMN

Burkhard BECHINGER
Professeur à l'Unistra

Year of creation of the group - 2000

https://rmnmc.chimie.unistra.fr

Le Bel Institute, 4th floor South

Nadia BOUAOUINA
email : nadia[at]unistra.fr
Phone : +33 (0)3 68 85 16 16

• Structural studies by liquid- and solid-state NMR of membrane proteins and membrane peptides : Bilayers, bicelles, micelles, oriented and non-oriented media.
• Dynamic Nuclear Polarization / solid state NMR of lipid membranes.
• Reconstitution of polypeptides in oriented bilayers and oriented bicelles.
• Bacterial Overexpression and purification of membrane proteins.
• Chemical synthesis of peptides.
• Study of the interactions of membrane polypeptides by NMR, optical and calorimetric techniques.
• Studies of the mechanism of action of cell penetrating peptides, peptide-mediated transport of nucleic acids, of viruses used in gene therapy (transfection and transduction) by solid-state NMR and by other biophysical techniques.
• Interactions of between polypeptides and between proteins and lipids within lipid bilayers, peptide oligomerisation in bilayers.
• Mechanisms of action and design of peptide antibiotics, pores and membrane channels.
• Study of amyloids.

Saad, A., Raya, J., Bechinger, B.
Structure and dynamics of the magainin 2 antimicrobial peptide in biomimetic lipid bilayers by solid-state NMR.
Biochemistry, 64, 4296-4308 (2025) - doi: 10.1021/acs.biochem.5c00467.
https://univoak.hal.science/UNIV-STRASBG/hal-05301598v1

De, K., Bryder, K., Aisenbrey, C., Mörgelin, M., S., Bechinger, B. 
Biophysical investigations of the membrane interactions of collagen VI-derived host defense peptides.
Biochimica et Biophysica Acta – Biomembranes, Special issue dedicated to Joachim Seelig, 1867,184448 (2025)- doi: 10.1016/j.bbamem.2025.184448. 
https://www.sciencedirect.com/science/article/pii/S0005273625000422?via%3Dihub
https://univoak.hal.science/hal-05324996

De, K., Guerinot, C., Charbonnel, N., Josse, J., Aisenbrey, C., Faure, S., Bechinger, B. 
Mechanism of action and membrane interactions of antibacterial quaternized triazolium peptoids.
ACS J Med Chem, 68, 26206-26217 (2025) - doi: 10.1021/acs.jmedchem.5c02254
https://univoak.hal.science/view/index/docid/5580835

Saad, A., Jiang, J.Q., Raya, J., Bechinger, B.
Phosphates Drive the Assembly of Fibers Made from the α-Helical Lentiviral Transduction Enhancer Vectofusin.
ACS Biomacromolecules, 26, 5130-5140 (2025) - doi: 10.1021/acs.biomac.5c00554
https://univoak.hal.science/search/index?q=10.1021%2Facs.biomac.5c00554+

Salnikov, E., Adélaïde, M., Ramos-Martín, F., Saad, A., Schauer, J., Cremanns, M., Rima, M., Aisenbrey, C., Oueslati, S., Naas, T., Pfennigwerth, N., Gatermann, S., Sarazin, S., Bechinger, B.^*, and D’Amelio, N.^* 
Cathelicidin-BF: A Potent Antimicrobial Peptide Leveraging Charge and Phospholipid Recruitment against Multidrug-Resistant Clinical Bacterial Isolates.
Journal of the American Chemical Society 147, 11199-11215 (2025) - doi: 10.1021/jacs.4c17821.
https://univoak.hal.science/UNIV-STRASBG/hal-05006267v1

Saad, A. and Bechinger, B. 
Solid-state NMR spectroscopy for structural studies of polypeptides in extended physiological membranes. Mini-review – perspectives article to honour the editor in chief of BBA Uli Brandt.
Biochimica et Biophysica Acta – Biomembranes, 1866, 184162, (2024) - doi: 10.1016/j.bbamem.2023.184162
https://univoak.hal.science/hal-05129571

Dussouillez, C., Lointier, M., Sebane, M.K., Fournel, S., Bechinger, B., Kichler, A.
N-terminal modification of an LAH4 derived peptide increases mRNA delivery in the presence of serum.
Journal of Peptide Science Mar 25:e3597 (2024) - doi: 10.1002/psc.3597 
https://hal.science/hal-04522663v1

Aisenbrey, C. and Bechinger, B.
Multimerization of the heptad repeat regions of the spike protein of SARS-CoV 2.
Biochimica et Biophysica Acta - Biomembranes 1866: 184259 (2024) – doi: 10.1016/j.bbamem.2023.184259.
https://cnrs.hal.science/hal-04670455

Saad, A. and Bechinger, B.
Solid-state NMR spectroscopy for structural studies of polypeptides and lipids in extended physiological membranes. (perspectives article)
Biochimica et Biophysica Acta – Biomembranes, 1866(2):184162 (2024) - doi: 10.1016/j.bbamem.2023.184162https://univoak.hal.science/hal-05129571v1

Roversi, D., Troiano, C., Salnikov, E., Giordano, L., Riccitelli, F., De Zotti, M., Casciaro, B., Loffredo, M.R., Park, Y., Formaggio, F., Mangoni, M.L., Bechinger, B. and Stella, L.
Effects of antimicrobial peptides on membrane dynamics: A comparison of fluorescence and NMR experiments.
Biophysical Chemistry 300:107060 (2023) - doi: 10.1016/j.bpc.2023.107060
https://www.sciencedirect.com/science/article/pii/S0301462223001114
https://univoak.hal.science/UNIV-STRASBG/hal-04184481v1

Bechinger, B.
Editor of the Special Issue: 50 years of the Fluid Mosaic Model of cell membranes.
Biochimica et Biophysica Acta - Biomembranes 1865:184181 (2023) - doi: 10.1016/j.bbamem.2023.184181
https://www.journals.elsevier.com/biochimica-et-biophysica-acta-biomembranes/special-issues

Salnikov E, Aisenbrey C, and Bechinger B
Lipid saturation and head group composition have a pronounced influence on the membrane insertion equilibrium of amphipathic helical polypeptides. 
Biochimica et Biophysica Acta, 1864(4):183844. (2022) - doi:10.1016/j.bbamem.2021.183844 
https://univoak.hal.science/hal-03602243v1

Aisenbrey C, and Bechinger B
Structure, interactions and membrane topology of HIV gp41 ectodomain sequences. Biochimica et Biophysica Acta, 1862(7):183274 (2020) - doi: 10.1016/j.bbamem.2020.183274
https://univoak.hal.science/hal-03015605v1

Aisenbrey C, Amaro M, Pospisil P, Hof M, and Bechinger B
Highly synergistic antimicrobial activity of Magainin 2 and PGLa peptides is rooted in the formation of supramolecular complexes with lipids.
Nature Scientific Reports 10(1):11652 (2020) – doi: 10.1038/s41598-020-68416-1
https://univoak.hal.science/hal-03003867v1

Aisenbrey C, Rifi O, and Bechinger B
Structure, membrane topology and influence of cholesterol interactions of the membrane proximal region - transmembrane helical anchor sequence of gp41 from HIV.
Scientific Reports, 10:22278 (2020) – doi: : 10.1038/s41598-020-79327-6
https://www.nature.com/articles/s41598-020-79327-6

Aisenbrey C, Marquette A, and Bechinger B
The mechanisms of action of cationic antimicrobial peptides refined by novel concepts from biophysical investigations. Book chapter in 'Antimicrobial Peptides: From Basics to Clinical Application', editor Katsumi Matsuzaki.
Adv Exp Med Biol., 1117, 33-64 (2019) - doi: 10.1007/978-981-13-3588-4_4.
https://link.springer.com/chapter/10.1007%2F978-981-13-3588-4_4 https://univoak.hal.science/hal-02323904v1

Salnikov ES, Aisenbrey C, Prokandt B, Brügger B, and Bechinger B
Structure, Topology, and Dynamics of Membrane-Inserted Polypeptides and Lipids by Solid-State NMR Spectroscopy: Investigations of the transmembrane domains of the DQ beta-1 subunit of the MHC II receptor and of the COP I protein p24. 
Frontiers in Molecular Biosciences, Structural Biology 6, article 83 (2019) - doi: 10.3389/fmolb.2019.00083
https://univoak.hal.science/hal-02323427v1

Salnikov ES, Anantharamaiah GM, and Bechinger B
Supramolecular organization of apolipoprotein A-I - derived peptides within disc-like arrangements.
Biophysical Journal 115(3):467-477 (2018) - doi: 10.1016/j.bpj.2018.06.026
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085177/(open access)

Bechinger B
DNP solid-state NMR spectroscopy on membranes.
Special issue on Dynamic Nuclear Polarization NMR, editor Vladimir K. Michaelis eMagRes. (2018) 
https://univoak.hal.science/hal-03034943v1