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Biophysique des membranes et RMN

Head of the research group

Burkhard BECHINGER
Professeur à l'Unistra

Year of creation of the group - 2000

Location

Le Bel Institute, 4th floor South

Secretarial and accounting services provided by

Emilie RANCHON
email : eranchon@unistra.fr
Phone : +33 (0)3 68 85 16 84

Permanent members

  • BECHINGER Burkhard
    Professeur à l'Unistra
    Phone: +33 (0)3 68 85 13 03
  • HIRSCHINGER Jérôme
    Chargé de recherche CNRS
    Phone: +33 (0)3 68 85 14 94
  • BERTANI Philippe
    Maître de conférences à l'Unistra
    Phone: +33 (0)3 68 85 65 22
  • AISENBREY Christopher
    Chargé de recherche CNRS
    Phone:     +33 (0)3 68 85 13 13
  • GLATTARD Elise
    Ingénieur de recherche CNRS
    Phone: +33 (0)3 68 85 17 34
  • RAYA Jésus
    Ingénieur de Recherche CNRS
    Phone: +33 (0)3 68 85 14 94

*works for several groups

  

Non-permanent members

PhD students

  • Kathakali DE
  • Jasmin SCHLAUCH
  • Romuald MANCA
  • Adrien GEBUS
  • Amanda NEVES DE SOUZA

Postdocs

  • Evgeniy SALNIKOV
  • Ahmad SAAD

Research topics

  • Structural studies by liquid- and solid-state NMR of membrane proteins and membrane peptides : Bilayers, bicelles, micelles, oriented and non-oriented media.
  • Dynamic Nuclear Polarization / solid state NMR of lipid membranes.
  • Reconstitution of polypeptides in oriented bilayers and oriented bicelles.
  • Bacterial Overexpression and purification of membrane proteins.
  • Chemical synthesis of peptides.
  • Study of the interactions of membrane polypeptides by NMR, optical and calorimetric techniques.
  • Studies of the mechanism of action of cell penetrating peptides, peptide-mediated transport of nucleic acids, of viruses used in gene therapy (transfection and transduction) by solid-state NMR and by other biophysical techniques.
  • Interactions of between polypeptides and between proteins and lipids within lipid bilayers, peptide oligomerisation in bilayers.
  • Mechanisms of action and design of peptide antibiotics, pores and membrane channels.
  • Study of amyloids.

Prof. Burkhard Bechinger talks about his research on antimicrobial peptides

Selection of key publications

Bechinger, B.
Editor of the Special Issue: 50 years of the Fluid Mosaic Model of cell membranes
Biochimica et Biophysica Acta - Biomembranes (submitted, 2023).
https://www.journals.elsevier.com/biochimica-et-biophysica-acta-biomembranes/special-issues

Salnikov E, Aisenbrey C, and Bechinger B
Lipid saturation and head group composition have a pronounced influence on the membrane insertion equilibrium of amphipathic helical polypeptides.
Biochimica et Biophysica Acta, 1864(4):183844. (2022) - doi:10.1016/j.bbamem.2021.183844
https://univoak.eu/islandora/object/islandora%3A143849

Aisenbrey C, and Bechinger B
Structure, interactions and membrane topology of HIV gp41 ectodomain sequences Biochim Biophys Acta 1862(7):183274 (2020) - doi: 10.1016/j.bbamem.2020.183274
https://univoak.eu/islandora/object/islandora:102827

Aisenbrey C, Amaro M, Pospisil P, Hof M, and Bechinger B
Highly synergistic antimicrobial activity of Magainin 2 and PGLa peptides is rooted in the formation of supramolecular complexes with lipids.
Nature Scientific Reports 10(1):11652 (2020) – doi: 10.1038/s41598-020-68416-1
https://univoak.eu/islandora/object/islandora:102830

Aisenbrey C, Rifi O, and Bechinger B
Structure, membrane topology and influence of cholesterol interactions of the membrane proximal region - transmembrane helical anchor sequence of gp41 from HIV
Scientific Reports, 10:22278 (2020) – doi: : 10.1038/s41598-020-79327-6
https://www.nature.com/articles/s41598-020-79327-6

Aisenbrey C, Marquette A, and Bechinger B
The mechanisms of action of cationic antimicrobial peptides refined by novel concepts from biophysical investigations.
Book chapter in 'Antimicrobial Peptides: From Basics to Clinical Application', editor Katsumi Matsuzaki.
Adv Exp Med Biol., 1117, 33-64 (2019) - doi: 10.1007/978-981-13-3588-4_4.
https://link.springer.com/chapter/10.1007%2F978-981-13-3588-4_4
https://univoak.eu/islandora/object/islandora:78459

Salnikov ES, Aisenbrey C, Prokandt B, Brügger B, and Bechinger B
Structure, Topology, and Dynamics of Membrane-Inserted Polypeptides and Lipids by Solid-State NMR Spectroscopy: Investigations of the transmembrane domains of the DQ beta-1 subunit of the MHC II receptor and of the COP I protein p24
Frontiers in Molecular Biosciences, Structural Biology 6, article 83 (2019) - doi: 10.3389/fmolb.2019.00083
https://univoak.eu/islandora/object/islandora:102824

Salnikov ES, Anantharamaiah GM, and Bechinger B
Supramolecular organization of apolipoprotein A-I - derived peptides within disc-like arrangements Biophysical Journal 115(3):467-477 (2018) - doi: 10.1016/j.bpj.2018.06.026
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085177/ (open access)

Bechinger B
DNP solid-state NMR spectroscopy on membranes.
Special issue on Dynamic Nuclear Polarization NMR, editor Vladimir K. Michaelis eMagRes. (2018) https://doi.org/10.1002/9780470034590.emrstm1558

Itkin A et al.
Structural characterization of the amyloid precursor protein transmembrane domain and its gamma‑cleavage site. ACS Omega 2, 6525-6534 (2017).

Salnikov ES et al.
Solid-State NMR Membrane topologies of the PGLa antimicrobial peptide and a transmembrane anchor sequence by DNP / solid-state NMR spectroscopy, Nat Scie Rep 6:20895 (2016).

Bechinger B
Domains stack up.
Nature Materials, 11, 1005-6 (2012).

Resende JM et al. .
The antibiotic heterodimeric distinctin by ssNMR.
PNAS, 106,16639 (2009).

Kichler A et al.
Histidine-rich Amphipathic Peptide Antibiotics Promote Efficient Delivery of DNA into Mammalian Cells.
PNAS, 100, 1564 (2003).

Photo gallery

A small overview of our research group