Chemistry – A European Journal

Abstract

We report the design and synthesis of ratiometric ¹⁹F-ATCUN (Xxx-Zzz-His) peptide probes for the selective and reversible detection of Ni²⁺ and Cu²⁺ by ¹⁹F NMR spectroscopy. In contrast to fluorescence-based approaches, ¹⁹F NMR enables background-free detection in biological media, due to the absence of fluorine in native biomolecules. The probes remain stable and functional in complex cell media (DMEM), enabling the detection of Cu²⁺ and Ni²⁺ by ¹⁹F NMR with a limit of detection (LOD) of 4 µM and quantification (LOQ) of 10 µM, relevant for studying copper-related pathologies.

Reference

A 19F‐ATCUN Peptide NMR Probe for Selective Cu2+ or Ni2+ Sensing in Complex Biological Media

Marie‐Julie Tilly, Enrico Falcone, Bruno Kieffer, Yannis‐Nicolas François, Peter Faller, Marine Desage‐El Murr, Laurent Raibaut

Chemistry – A European Journal, 2025-08-19 - DOI: 10.1002/chem.202501784

Contacts

Laurent Raibaut & Peter Faller (team BCB), Institut de Chimie de Strasbourg, UMR 7177.

Marine Desage-El Murr (team OMECA), Institut de Chimie de Strasbourg, UMR 7177.