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Séminaire David P. Giedroc

June 20, 2018
Salle Mauve, Institut de Chimie

Seminar,Wednesday 20st June at 16h3O
Esplanade "salle mauve"

"Mechanisms of Zinc Metallostasis in Bacterial Pathogens"

David P. Giedroc

Department of Chemistry, Indiana University, Bloomington, IN 47405-7102, USA
Chair, Editorial Board, Metallomics (RSC)  


First-row late d-block transition metals from Mn to Zn play critical and distinct roles in many aspects of cellular metabolism. In bacterial pathogens, metalloregulation of transcription underscores physiological adaptation to host-mediated transition metal starvation and toxicity, required to maintain metal homeostasis (metallostasis). In zinc (Zn) homeostasis, a pair of metal-sensing transcriptional repressors regulate the transcription of metal uptake and efflux transporters, where Zn typically allosterically activates or inhibits, respectively, DNA operator-promoter binding.  We have long-standing interests in the molecular underpinnings of allostery in metalloregulatory proteins, transition metal transporter function and the impact that dysregulation of the process has on cellular physiology.  In this presentation, I will discuss recent efforts to understand allostery in Zn-regulated repressors using NMR spectroscopy and companion biophysical methods, primarily focused in the Zn efflux regulator from Staphylococcus aureus, CzrA, and a Zn uptake regulator adhesin-competence repressor (AdcR) from the Gram-positive respiratory pathogen, Streptococcus pneumoniae.  These studies provide new and general insights into the evolution of allostery in these large regulatory protein families, and the role of host-imposed Zn toxicity on microbial physiology.

For more details about the research of David P. Giedroc seehttp://www.indiana.edu/~dpglab/